Molecular Biology Services

Gene Synthesis Service

Compared to conventional molecular cloning methods, gene synthesis saves time and effort in obtaining target genes. Four service formats cover simple to complex sequences – from 100 bp fragments to constructs of up to 200 kb.

Standard Gene Synthesis ProLongGene DNA Fragment Single-Stranded DNA

■Gene Synthesis

Standard Gene Synthesis

Gene Synthesis

Compared to conventional molecular cloning methods, gene synthesis saves time and effort in obtaining target genes. Our standard gene synthesis service offers personalized synthesis plans and services tailored to different lengths and application requirements. Simply provide the DNA sequence you need to synthesize, and we assure prompt delivery of the ideal plasmid containing your target gene.

A cost-effective solution through Tsingke’s comprehensive nucleic acid synthesis industrial chain.

Insert image hereTechnician working with automated equipment (photo)images/molecular-biology/standard-gene-synthesis-main.jpg

Highly CustomizedFrom simple sequences to complex sequences.

Free 160+ VectorsClone into over 160 common commercial vectors free of charge.

100% Sequence AccuracyGuaranteed with Sanger sequencing and NGS.

Free Codon OptimizationCodon optimization is provided free of charge.

Fast TurnaroundAs fast as 5 days.

Gene lengthUp to 10 kb

Standard delivery1~4 μg plasmid

TurnaroundAs fast as 5 days

Workflow

Sequence Analysis & Codon Optimization

Oligo Design & Synthesis

ConstructionOverlap PCR & gene assembly

Quality ControlQC for NGS & Sanger sequencing

DeliveryShip the plasmid containing your gene insert

Service Details

Standard Gene Synthesis – Turnaround time5 length ranges

Length	Turnaround time (calendar days)*
< 1.5 kb	5~8
1.5 kb~3 kb	7~11
3 kb~6 kb	10~15
6 kb~8 kb	15~22
> 8 kb	Evaluation

VectorAny vector. If not specified otherwise, default to using pUC57.

*Turnaround time applies to simple sequences only and may change with the complexity of the gene sequence. For an accurate estimate, email [email protected].

Deliverables

Lyophilized plasmid DNA1 tube (about 1~4 μg per tube).

QC filesSequencing map (.ab1 file); target sequence (.seq file); COA report (electronic).

Optional identificationAdditional identification by restriction endonuclease digestion is available (please specify your request before placing your order).

Value Added Services

Gene cloningWe can clone sequences into any vector, offering over 160 common commercial vectors free of charge.

Plasmid preparationFrom microgram to gram-scale plasmid DNA preparation, catering to scientific research as well as industrial requirements.

ProLongGene

Gene Synthesis

Tsingke leads in addressing challenges in gene synthesis, focusing on advancing long-fragment synthesis and assembly technologies. In gene synthesis, where chemical methods limit lengths to 200 nucleotides, we excel in achieving kilobase to megabase-level genes, even entire genomes, through advanced in vitro assembly technologies. With extensive expertise and ongoing innovation, we’ve surpassed gene synthesis boundaries.

Our breakthroughs include mastering yeast assembly for multiple segments and large fragments, enabling precise and efficient one-step assembly of DNA fragments in Saccharomyces cerevisiae. We proficiently produce industrial-scale 50 kb large fragment DNA, demonstrating the capability to deliver fragments up to 200 kb.

Insert image hereAutomated liquid-handling workstation (photo)images/molecular-biology/prolonggene-main.jpg

Up to 200 kbAchieved construction as long as 200 kb.

Yeast Assembly SystemIndependently developed yeast assembly system.

One-Step AssemblyOne-step assembly of DNA fragments in Saccharomyces cerevisiae.

100% Sequence AccuracyGuaranteed.

LengthUp to 200 kb

Standard delivery1~4 μg plasmid

VectorpCC1413

Workflow

Service Details

ProLongGene – Turnaround time1 item

Length	Turnaround time (calendar days)	Vector
> 10 kb	Evaluation	pCC1413

Deliverables

Lyophilized plasmid DNA1 tube (about 1~4 μg per tube).

QC filesSequencing map (.ab1 file); target sequence (.seq file); COA report (electronic).

Applications

Genome SynthesisGene StorageMicrobial Strain Modification (primarily used for microbial cells)Metabolic Engineering (used for host cells)Stem Cell Therapy

DNA Fragment

Gene Synthesis

Tsingke’s DNA Fragment Service provides double-stranded DNA fragments, accommodating lengths of up to 1,200 base pairs. This service caters to the requirements of straightforward gene construction or modification, empowering you to accelerate your research and development activities for various applications such as antibody development, CRISPR-mediated genome editing, and NGS controls.

Insert image hereLaboratory with automated instruments (photo)images/molecular-biology/dna-fragment-main.jpg

High Yield

Low Error Rate (Column-Based)

High Delivery Rate

High Coupling Efficiencies

Free Codon Optimization

Length100 bp to 1.2 kb

Delivery500 ng or 1 μg PCR products

Turnaround2 to 3 days

Service Details

DNA Fragment – Turnaround time1 item

Length	Turnaround time (calendar days)
100 bp~1.2 kb	2~3

Deliverables

Lyophilized DNA1 tube (about 500 ng per tube).

QC filesCOA report (electronic).

Optional identificationAdditional identification by Sanger sequencing is available (please specify your request before placing your order).

Applications

Gene CloningInsert specific DNA sequences into a target vector for expression.

Molecular LabelingBe employed as molecular labels, for instance, in experiments like Southern blot, Northern blot, and Western blot.

Gene Mutation ResearchBy introducing specific DNA fragments, gene knock-in, knock-out, or mutation studies can be conducted to understand gene functions.

Molecular DiagnosisDetecting the presence or absence of specific genes through PCR.

Genome EditingCRISPR-Cas9 for genome editing and modification.

Single-Stranded DNA (ssDNA)

Gene Synthesis

Recent research highlights the effectiveness of single-stranded DNA (ssDNA) as a homology-directed repair donor template in CRISPR gene editing. Compared to double-stranded DNA (dsDNA), using ssDNA as a template for homologous recombination will achieve a 9-fold higher CRISPR/Cas9 gene knock-in efficiency compared to dsDNA, and ssDNA is versatile in various biological reactions, especially in DNA nanotechnology.

Tsingke now provides top-notch, sequence-validated ssDNA to enhance the efficiency of your CRISPR experiments.

Insert image hereTechnician operating a laboratory instrument (photo)images/molecular-biology/ssdna-main.jpg

High AccuracySequence-validated ssDNA.

High Editing Efficiency9-fold higher CRISPR/Cas9 knock-in efficiency than dsDNA.

Low Cytotoxicity

Wide Synthesis RangeUp to 6000 nt.

Length100 nt to 6000 nt

DeliveryLyophilized product powder

Service Details

Single-Stranded DNA – Turnaround time6 length ranges

Length (nt)	Turnaround time (business days)
101~240	10
241~1000	15
1001~3000	22
3001~4000	28
4001~5000	32
> 5000	Evaluation

Deliverables

Lyophilized product powder

QC filesSequencing map (.ab1 file); target sequence (.seq file); COA report (electronic).

Applications

In CRISPR and CRISPR-Cas9 genome editing experiments, long ssDNA serves as a potent donor template, enhancing both insertion and gene replacement efficiency. Its applications extend to single-strand conformation polymorphism, in vitro transcription studies, nucleic acid enzyme S1 mapping, probe preparation, labeling, and differential hybridization. Beyond experiments, ssDNA plays a role in DNA nanotechnology, serving as a scaffold for drug delivery, molecular diagnostics, DNA-based data storage, and diverse nanoscale applications. With a lower risk of random integration, ssDNA is particularly suitable for gene editing in primary cells, stem cells, and the creation of genetically modified animal models.

Gene Cloning Service

PCR cloning and subcloning are techniques commonly used in molecular biology to amplify and manipulate DNA fragments for gene cloning, sequencing and functional analysis, while site-directed mutagenesis is a powerful tool to efficiently enhance the expression, traits, and characterization of the targeted protein. Tsingke offers a comprehensive service encompassing gene synthesis, vector construction, and cloning, which ensures heightened efficiency throughout the entire process.

PCR Cloning & Subcloning Mutagenesis

■Gene Cloning

Insert image herePCR instruments (photo)images/molecular-biology/cloning-overview-main.jpg

PCR Cloning & Subcloning

Gene Cloning

PCR cloning and subcloning are common molecular biology techniques for amplifying and manipulating DNA fragments, including gene cloning, sequencing, and functional analysis. PCR cloning uses the polymerase chain reaction to amplify a specific DNA fragment without requiring specific restriction enzyme sites. Subcloning involves transferring a DNA fragment from one vector to another.

Tsingke offers a one-stop service ranging from gene synthesis and vector construction to cloning and subcloning. Our advanced technology and extensive experience ensure high-quality cloning services.

Insert image hereTechnician working at a clean bench (photo)images/molecular-biology/pcr-cloning-main.jpg

100% Sequence AccuracyGuaranteed with Sanger sequencing and NGS.

No LimitNot limited by restriction enzyme cutting sites.

CustomizableClone the target gene fragment at any site in any vector system – any site, any vector.

One-Stop SolutionFrom gene synthesis and vector construction to cloning and subcloning.

Service Details

PCR Cloning & Subcloning – Turnaround time5 length ranges

Length	Turnaround time (calendar days)
< 1.5 kb	7~10
1.5 kb~3 kb	7~11
3 kb~6 kb	10~15
6 kb~8 kb	13~18
> 8 kb	Evaluation

Deliverables

Lyophilized plasmid DNA1 tube (about 1~4 μg per tube).

QC filesSequencing map (.ab1 file); target sequence (.seq file); COA report (electronic).

Applications

Introduction of MutationsGene Library ConstructionDNA LabelingGene Construction for Protein Expression

Mutagenesis

Gene Cloning

Site-directed mutagenesis, a molecular biology technique, could deliberately introduce precise changes or mutations into a specific DNA sequence. This includes base insertions, deletions, and point mutations. The technology of site-directed mutagenesis is now extensively employed in the investigation of protein functional site structures, optimization of enzyme activity, understanding of DNA component functions and interactions, as well as applications in gene therapy and other research areas.

Tsingke provides specialized and cost-effective site-directed mutagenesis services, precisely introducing your required mutations for any gene template and any target number of mutations. This facilitates increased experimental efficiency and cost-effectiveness for your research endeavors.

Insert image hereModern molecular biology laboratory (photo)images/molecular-biology/mutagenesis-main.jpg

100% Sequence AccuracyGuaranteed with Sanger sequencing and NGS.

Cost-EffectiveAll mutations found within a 30-base region will be defined as one whole mutation.

Unlimited SitesIntroduce your mutations at any site.

Mutation typesInsertions · deletions · point mutations

Mutation counting30 bp region = 1 mutation

Service Details

Mutagenesis – Turnaround time5 length ranges

Length	Turnaround time (calendar days)
< 1.5 kb	7~10
1.5 kb~3 kb	7~11
3 kb~6 kb	10~15
6 kb~8 kb	13~18
> 8 kb	Evaluation

Deliverables

Lyophilized plasmid DNA1 tube (about 1~4 μg per tube).

QC filesSequencing map (.ab1 file); target sequence (.seq file); COA report (electronic).

Mutation countingAny mutations identified within a 30-base region will be considered as a single comprehensive mutation.

Applications

Disease ResearchGene Function StudiesDrug DevelopmentGenetic Engineering

Plasmid Preparation

Research grade and transfection grade plasmid preparation, from microgram to gram scale, with high supercoil content and low endotoxin level.

Plasmid Preparation

■Plasmid Preparation

Plasmid Preparation

Plasmids are small, circular DNA molecules used as vectors in DNA recombination. In molecular biology, the target gene is inserted into a plasmid’s multiple cloning sites, forming a recombinant plasmid. This can enter recipient cells, undergo replication and expression, and transfer to progeny cells during host cell division. Plasmid quantity and purity may vary, emphasizing the importance of obtaining high-quality plasmids for successful experiments.

Tsingke offers research grade and transfection grade plasmid preparation services to satisfy different downstream applications’ requirements.

Insert image herePipetting a plasmid sample (photo)images/molecular-biology/plasmid-prep-main.jpg

One-Stop SolutionFrom gene synthesis and vector construction to plasmid preparation.

High Industrial StandardHigh supercoil content, low endotoxin level.

Flexible CustomizedProduce plasmid DNA from microgram to gram level.

Scaleμg ~ g

GradesResearch · Transfection

Choose a Grade

Research Grade

Obtain increased quantities of plasmid (default concentration 1000 ng/μL).

Supercoil content > 50%

Transfection Grade

Removes endotoxin, increases supercoil ratio, and achieves highly efficient cell transfection.

Supercoil content > 85%
Endotoxin < 0.1 / < 0.01 / < 0.005 EU/μg
LAL test and NGS contamination check

Workflow

Sequence templateShip target plasmid to us, or use a template previously synthesized by Tsingke

Plasmid preparation

Quality control

DeliveryShip the plasmid with your template sequence inserted

Service Details

Turnaround time by volume – Research Grade vs. Transfection Grade12 volumes

Volume	Research Grade (calendar days)*	Transfection Grade (calendar days)*
100 μg	5	5
200 μg	6	6
500 μg	8	8
1 mg	8	8
2 mg	9	9
3 mg	10	10
4 mg	11	11
5 mg	12	12
10 mg	15	15
20 mg	16	17
50 mg	17	19
100 mg	21	22

*Opt for gene synthesis alongside plasmid preparation and save 2 days on turnaround time.

Quality Control

Quality control specifications10 items

Items	Method	Research Grade	Transfection Grade
Appearance	Visual inspection	Colorless liquid of pure transparency	Colorless liquid of pure transparency
A260/280 ratio	UV absorbance	1.8~2.0	1.8~2.0
Supercoil content	Agarose gel electrophoresis	> 50%	> 85%
Concentration	UV absorbance	90%~110%	90%~110%
Residual DNA	Agarose gel electrophoresis	Not visible	Not visible
Residual RNA	Agarose gel electrophoresis	Not visible	Not visible
Restriction enzyme analysis	Agarose gel electrophoresis	Adjustable according to requirement	Adjustable according to requirement
Sequence verification	Sanger sequencing	Consistent with the confirmed plasmid sequence	Consistent with the confirmed plasmid sequence
Endotoxin	Limulus amebocyte lysate (LAL) test	—	Endotoxin ≤ standard (< 0.1 / < 0.01 / < 0.005 EU/μg)
Exogenous contamination detection	NGS (depth > 30×)	—	Genomic DNA < 1%; other DNA contamination < 0.1%

Deliverables

Lyophilized plasmid DNAPrepared lyophilized plasmid DNA.

Sequencing map.ab1 file.

COA reportElectronic.

Resource Center

Codon optimization case studies and a selection of publications citing Tsingke gene synthesis services.

Codon Optimization Citation

■Resource Center

Codon Optimization

Case studies

Codon optimization is provided free of charge with Tsingke’s gene synthesis service. The two cases below show how it affects protein expression and solubility.

Case 1 – Enhanced protein expression after codon optimization

The target protein 16.8 kDa was not expressed before codon optimization, but was highly expressed after codon optimization.

Target protein16.8 kDa

ResultHigh expression after optimization

Case 2 – Codon optimization enhances protein soluble expression

The target protein 100 kDa was expressed in inclusion bodies before codon optimization, and the supernatant was 75% soluble after codon optimization.

Target protein100 kDa

Result75% soluble in supernatant

Citation

Publications

Tsingke has been cited in numerous journals such as Nature, Cell, Science, and others, totaling thousands of citations.

Below are some publication details from customers who have placed gene synthesis service orders.

Selected publications10 items

No.	Journal	Impact Factor	Title	DOI
1	Nature Biotechnology	68.164	Strand-selective base editing of human mitochondrial DNA using mitoBEs	10.1038/s41587-023-01791-y
2	Science	56.9	Structure of the human PKD1-PKD2 complex	10.1126/science.aat9819
3	Science	56.9	Enhancing rice panicle branching and grain yield through tissue-specific brassinosteroid inhibition	10.1126/science.adk8838
4	Mol Cancer	41.44	A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR	10.1186/s12943-023-01719-9
5	Circulation	39.9	Cannabinoid Receptor 2-Centric Molecular Feedback Loop Drives Necroptosis in Diabetic Heart Injuries	10.1161/CIRCULATIONAHA.122.059304
6	Journal of Infection	38.637	MAMDC2, a gene highly expressed in microglia in experimental models of Alzheimer’s Disease, positively regulates the innate antiviral response during neurotropic virus infection	10.1016/j.jinf.2021.12.004
7	Signal Transduction and Targeted Therapy	38.1196	UBQLN1 mediates sorafenib resistance through regulating mitochondrial biogenesis and ROS homeostasis by targeting PGC1β in hepatocellular carcinoma	10.1038/s41392-021-00594-4
8	Signal Transduction and Targeted Therapy	38.1196	O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N6-methyladenosine-dependent manner	10.1038/s41392-023-01316-8
9	Signal Transduction and Targeted Therapy	38.1196	Excessive branched-chain amino acid accumulation restricts mesenchymal stem cell-based therapy efficacy in myocardial infarction	10.1038/s41587-023-01791-y
10	Signal Transduction and Targeted Therapy	38.1196	Targeting carnitine palmitoyl transferase 1A (CPT1A) induces ferroptosis and synergizes with immunotherapy in lung cancer	10.1126/science.aat9819

Technology & Quality

Technology platforms, manufacturing capacity and quality credentials of our strategic partner Shanghai Yeasen Biotechnology.

Technology Platform ZymeEditor Molecular Diagnostic NGS mRNA Hieff Trans™UCF.ME mRNAtools Factory DMF Filing

■Company Profile

Company Positioning & Company Culture

Shanghai Yeasen Biotechnology

To serve the life science industry, and to be innovators of life science tools.

Life Science Industry Pyramid

Scientific researchLife science research

DetectionMolecular diagnosis, colloidal gold, fluorescence, biochemical detection, chemiluminescence, POCT…

TherapyAntibody drugs, nucleic acid drugs, antibodies, CAR-T, mRNA vaccine, adenovirus vaccine, inactivated vaccine…

ToolsReagents, consumables, instruments, CRO, CDMO

Mission, Vision & Value

To enable success of our customers. Together to make a healthier and brighter world.

Innovating in the life science tools industry.

Integrity
Altruism
Innovation
Collaboration
Endeavour

Company Location & Customers

Shanghai Yeasen Biotechnology

Research and development centers, manufacturing bases and warehouses in China, Singapore and the USA serve more than 20,000 clients and individual labs.

Company Location

Insert image hereWuhan site (photo)images/molecular-biology/yeasen-wuhan-site.jpg

Shanghai, China

Enzyme Evolution RDC
Protein/Antibody RDC
IVD RDC
Nucleic acid RDC
NGS RDC
2 manufacture bases

Wuhan, China

Ultra Clean Factory for Molecular Enzyme
mRNAtools manufacture base
Biolink Internet Center

Singapore

SG Warehouse

USA

Maryland Business and Warehousing Center
Overseas R&D Center (under preparation)

Customers

Customers20,000+ clients & individual labs

University LaboratoriesHospitals & Research InstitutesNGS Service ProvidersIVD Test Kit CompaniesClinical LaboratoriesPharmaceutical CompaniesCRO/CDMO Companies

■Technology Platforms

Technology Platform

Shanghai Yeasen Biotechnology

Yeasen has built six technical platforms, and built an innovation system covering the underlying technology and applied technology.

Directed Evolution & Rational Design of Molecular Enzymes Platform

Rational design of molecular enzyme
Directed evolution of molecular enzyme based on ultra-high-throughput single-cell sorting system

High Density Fermentation & Ultra-clean Protein Purification Platform

Molecular enzyme high density fermentation technology
Ultra-clean molecular enzyme production technology (DNA-free, DNase/RNase free)

Molecular IVD Platform

3D-Optimizing of molecular diagnostic reagent
Freeze-drying technology for diagnostic preparations

NGS Library Preparation Platform

Enzymatic digestion method for NGS library preparation
Ultra-fast RNA library construction technology

mRNA Preparation Platform

mRNA stock solution preparation and analysis technology
mRNA LNP preparation and evaluation technology

High-quality Monoclonal Antibody Platform

Hybridoma monoclonal antibody preparation technology
Phage display technology
Single B cell antibody preparation technology

Rational Design & Directed Evolution

ZymeEditor Platform

ZymeEditor Protein Directed Evolution and Rational Design Platform.

Insert image hereRational design and directed evolution workflow for engineered enzymesimages/molecular-biology/yeasen-rational-design.jpg

Performance improvementRapid improvement of the key performance of molecular enzymes including fidelity, activity, sensitivity, specificity, thermostability and substrate binding capacity to meet the application needs.

Faster evolution, lower costRapid enrichment of beneficial mutation sites, shortening the evolutionary cycle, increasing the success rate of screening and significantly reducing costs.

Single-cell sorting chip — Automated high-throughput single-cell sorting and isolation

Molecular Diagnostic Platform

Yeasen

Highlights: 3D-Optimizing of molecular diagnostic reagents and freeze-drying technology.

Advantages

3D-OptimizingTo provide our customers with selected enzymes, optimized reaction buffer and pre-designed primers/probe in compliance with PCR\LAMP technology.

Freeze-dryingTo remove more than 97% of the water and high success rate of freeze-drying.

Comprehensive applicationsIn vitro diagnosis / animal disease / early tumor screening / food safety detection, etc.

Molecular diagnostic reagents (photo) — Molecular diagnostic reagents

Freeze-drying beads (photo) — Freeze-drying beads

NGS Platform

Yeasen

Highlights: fast enzymatic digestion method for DNA library preparation · ultra-fast RNA library preparation · co-library technology that can distinguish between DNA and RNA sources.

Insert image hereNGS platform overview – library prep technologies and application fieldsimages/molecular-biology/yeasen-ngs-platform.jpg

PerformanceShort time-consuming, high efficiency, high fidelity and high stability.

ApplicationsTumor diagnosis, genetic disease screening and microbial infection detection (mNGS).

Data analysisSupporting bioinformatic analysis and data mining.

CompatibilityCompatible with MGI and Illumina sequencers.

mRNA Preparation Platform

Yeasen

Post-transcriptional capping and co-transcriptional capping routes, supported by mRNA stock solution preparation and analysis technology and mRNA LNP preparation and evaluation technology.

Technologies

mRNA stock solution preparation and analysis technologyThe mRNA yield can reach 10 g/L, the purity can reach more than 90%, and the capping rate can reach more than 95%. mRNA integrity detection, capping rate detection, and Poly(A) tail length detection.

mRNA LNP preparation and evaluation technologyMastering a series of detection techniques from in vitro physical characterization, cell level evaluation to animal level evaluation.

Hieff Trans™ Advanced R&D Platform

Yeasen

AI-powered novel transfection reagent development platform – core R&D strengths powered by high-throughput compound library screening.

AI-Driven Molecular Design PlatformUtilizing artificial intelligence (AI)-based molecular dynamics simulations and high-throughput virtual screening, this platform accurately predicts the structure-function relationships of transfection reagent molecules, rapidly identifying optimal modification sites. By simulating interactions between transfection reagents and cell membranes, the platform optimizes the ratio of cationic density to hydrophobic groups, effectively reducing cytotoxicity while enhancing transfection efficiency.

High-Throughput Biological Validation PlatformIntegrated with automated cell culture and flow cytometry systems, this platform enables high-throughput evaluation of transfection efficiency and cytotoxicity, accelerating the translation of candidate molecules from lab-scale discovery to industrial production. Tailored to specific application scenarios, the platform has successfully screened optimal transfection reagents from over ten thousand candidate compounds and completed three successful pilot production batches.

Screening of modified molecules for Hieff Trans™ novel transfection reagents.

■Qualification

UCF.ME: Ultra Clean Molecular Enzyme Factory

Qualification

The molecular enzymes were manufactured through high density fermentation and ultra-clean production process ensuring the cleanliness (DNA/RNA free, endotoxin free, sterile etc.) of enzymes following the ISO13485 quality management system.

Fermentation equipment in the ultra-clean enzyme factory (photo)

Closed SystemThe enzymes are produced in a closed system with sterile tanks and tubing, to reduce the potential environmental contamination.

Environmental ControlThe factory is equipped with a series of Class D, Class C, Class B and local Class A cleanroom to prevent kinds of contaminants.

Ultra Clean ProcessUnique purification technology with more than ten procedures to improve and guarantee the cleanliness and function of enzymes.

Large-scale ProductionIndustrial-grade facilities and chemical analysis equipment. Five independent production lines equipped with two 100L, one 500L and two 1,500L fermentation facilities and matched purification systems.

Stable BatchControlled procedures are executed to ensure the intra- and inter-batch consistency, and to promote high-standard, large-scale, stable preparation and long-term storage of molecular enzymes.

mRNAtools Manufacture Factory

Qualification

With an area of more than 5,000 square meters, the plant is equipped with automatic fermentation equipment and industrial-grade purification and freeze-drying facilities.

Insert image heremRNAtools factory – cleanroom corridor, fermentation and purification equipment (photo)images/molecular-biology/yeasen-mrnatools-factory.jpg

GMP GradeThe plant is designed and constructed in accordance with GMP standards for pharmaceuticals, and in compliance with the ISO13485:2016 quality management system.

Product PackageRaw and auxiliary materials including enzymes, nucleotides and SAM are provided for downstream mRNA vaccine production and drug preparation to meet the high standards for vaccine production and certification registration requirements.

High Production CapacityThe supply capacity of mRNA vaccine raw materials will be increased from 1 billion doses/year to 10 billion doses/year.

Area5,000+ m²

Quality systemISO13485:2016

DMF Filing

Qualification

Completed DMF (drug master file) filing of several products.

DMF filings10 items

S/N	Product Name	Application Number
1	T7 RNA Polymerase	MF037035
2	UltraNuclease GMP-grade	MF037298
3	mRNA Vaccinia Capping Enzyme GMP-grade	MF037433
4	mRNA Cap 2’-O-Methyltransferase GMP-grade	MF037434
5	Pyrophosphatase, Inorganic GMP-grade	MF037545
6	Murine RNase inhibitor GMP-grade	MF037546
7	BspQI GMP-grade	MF037607
8	Deoxyribonuclease I (DNaseI) GMP-grade	MF037744
9	Salt Active UltraNuclease GMP-grade	MF037815
10	PEI Transfection Reagent	MF038217

Service Overview

Gene Synthesis Service

Gene Cloning Service

Plasmid Preparation

Resource Center

Technology & Quality

Why GBT Molecular Biology Services

100% sequence accuracy

160+ free vectors

Free codon optimization

100 bp to 200 kb

Fast turnaround

One-stop, μg to g

Gene Synthesis Service

■Gene Synthesis

Standard Gene Synthesis

Workflow

Service Details

Deliverables

Value Added Services

ProLongGene

Workflow

Service Details

Deliverables

Applications

DNA Fragment

Service Details

Deliverables

Applications

Single-Stranded DNA (ssDNA)

Service Details

Deliverables

Applications

Gene Cloning Service

■Gene Cloning

PCR Cloning & Subcloning

Service Details

Deliverables

Applications

Mutagenesis

Service Details

Deliverables

Applications

Plasmid Preparation

■Plasmid Preparation

Plasmid Preparation

Choose a Grade

Research Grade

Transfection Grade

Workflow

Service Details

Quality Control

Deliverables

Resource Center

■Resource Center

Codon Optimization

Case 1 – Enhanced protein expression after codon optimization

Case 2 – Codon optimization enhances protein soluble expression

Citation

Technology & Quality

■Company Profile

Company Positioning & Company Culture

Life Science Industry Pyramid

Mission, Vision & Value

Company Location & Customers

Company Location

Shanghai, China

Wuhan, China

Singapore

USA

Customers

■Technology Platforms

Technology Platform

Directed Evolution & Rational Design of Molecular Enzymes Platform

High Density Fermentation & Ultra-clean Protein Purification Platform

Molecular IVD Platform

NGS Library Preparation Platform

mRNA Preparation Platform

High-quality Monoclonal Antibody Platform

Rational Design & Directed Evolution